Sporulation in Bacillis subtilis. Biochemical changes
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چکیده
منابع مشابه
Sporulation in bacillus subtilis. Antigenic changes during spore formation.
1. Antisera, prepared against extracts of cells and spores of Bacillus subtilis, were used in immunoelectrophoretic studies of the changes occurring in cell extracts during the course of spore formation. 2. At least 15 antigens could be detected in vegetative-cell extracts by the antiserum prepared against cell extracts and at least seven could be demonstrated in spore extracts by the homologou...
متن کاملBiochemical changes occurring during sporulation in Bacillus species.
is too fast to be measured by the methods used. The second step, which involves the acylation of the enzyme and the concomitant liberation of pnitrophenol, is characterized by the rate constant k2= 3-15 sec.-'. The third step involves the liberation of acetate and reactivation of the enzyme; the rate of this reaction is defined by kI3= 0-0254 sec.-'. 2. The rate constant k2 for the second step ...
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The effects of nutritional and environmental conditions on sporulation and the properties of the spore have been extensively studied with the hope that these may provide a clue to the mechanism of dormancy and resistance of the bacterial endospore. However, there is no general agreement and our knowledge of these is still limited. The extensive literature dealing with the various aspects of spo...
متن کاملGenetic and biochemical analyses of sensor kinase A in Bacillus subtilis sporulation.
Temporal and spatial regulation of gene expression during endospore formation in Bacillus subtilis prompted us to investigate the molecular mechanisms that coordinate the phosphorelay. We targeted KinA for random mutagenesis. In addition, we constructed KinA-GFP transcriptional fusions for verification, via fluorescence. Four distinct types of sporulation-defective mutants were identified as in...
متن کاملSporulation of Bacillus subtilis in Continuous Culture
Sporulation of Bacillus subtilis 168 was studied in chemostat cultures. Sporulation occurred at high frequency under limitation of growth by glucose or the nitrogen source in minimal medium, whereas rates of sporulation were low for Mg'+, phosphate, citrate, or tryptophan limitation. Sporulation was found at all growth rates tested, and the incidence of spores increased with decrease in growth ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1968
ISSN: 0306-3283
DOI: 10.1042/bj1090811